| pitx2a | Stage | Hours | ZFIN expression range | lateral mesoderm | lateral plate mesoderm | Rohon-Beard neuron | heart primordium | floor plate diencephalon | brain | diencephalon | epithalamus | heart rudiment | pharyngeal epithelium | unspecified |
| Zygote | 1-cell | 0 | ||||||||||||
| Cleavage | 2-cell | 0.75 | ||||||||||||
| 4-cell | 1.0 | |||||||||||||
| 8-cell | 1.25 | |||||||||||||
| 16-cell | 1.5 | |||||||||||||
| 32-cell | 1.75 | |||||||||||||
| 64-cell | 2.0 | |||||||||||||
| Blastula | 124-cell | 2.25 | ||||||||||||
| 256-cell | 2.5 | |||||||||||||
| 512-cell | 2.75 | |||||||||||||
| 1k-cell | 3.0 | |||||||||||||
| High | 3.33 | |||||||||||||
| Oblong | 3.66 | |||||||||||||
| Sphere | 4.0 | |||||||||||||
| Dome | 4.33 | |||||||||||||
| 30% epiboly | 4.66 | |||||||||||||
| Gastrula | 50%-epiboly | 5.25 | ||||||||||||
| Germ-ring | 5.66 | |||||||||||||
| Shield | 6.0 | |||||||||||||
| 75% epiboly stage | 8.0 | |||||||||||||
| 90% epiboly | 9.0 | I | I | |||||||||||
| bud stage | 10.0 | I | I | |||||||||||
| Segmentation | 1-4 somites | 10.33 | I | I | ||||||||||
| 5-9 somites | 11.66 | I | I | |||||||||||
| 10-13 somites | 14.0 | I | I | |||||||||||
| 14-19 somites | 16.0 | |||||||||||||
| 20-25 somites | 19.0 | |||||||||||||
| 26+ somites | 22.0 | I | ||||||||||||
| Pharyngula | Prim-5 | 24.0 | I | I | ||||||||||
| Prim-15 | 30.0 | I | I | I | ||||||||||
| Prim-25 | 36.0 | I | I | I | ||||||||||
| High-pec | 42.0 | I | I | I | ||||||||||
| Hatching | Long-pec | 48.0 | I | |||||||||||
| Pec-fin | 60.0 | I |
| Evidence for Interaction | Embryos injected with pitx2a displayed ectopic activation of pitx2a in both marginal and animal pole cells at 30% epiboly |
| Target Gene | pitx2a |
| Upstream Gene | pitx2a |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 4.66 |
| Location of Interaction in evidence | animal pole cells |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | H. Joseph Yost |
| Journal | Development |
| Year | 2000 |
| Vol | 127 |
| Start Page | 1081 |
| Evidence for Interaction | Embryos injected with pitx2a displayed ectopic activation of pitx2a in both marginal and animal pole cells at 30% epiboly |
| Target Gene | pitx2a |
| Upstream Gene | pitx2a |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 4.66 |
| Location of Interaction in evidence | animal pole cells |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | H. Joseph Yost |
| Journal | Development |
| Year | 2000 |
| Vol | 127 |
| Start Page | 1081 |
| Evidence for Interaction | gsc-injected embryos displayed activation of pitx2a and pitx2c in both marginal and animal pole cells. |
| Target Gene | pitx2a |
| Upstream Gene | gsc |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 4.66 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | H. Joseph Yost |
| Journal | Development |
| Year | 2000 |
| Vol | 127 |
| Start Page | 1081 |
| Evidence for Interaction | gsc-injected embryos displayed activation of pitx2a in both marginal and animal pole cells |
| Target Gene | pitx2a |
| Upstream Gene | gsc |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 4.66 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | H. Joseph Yost |
| Journal | Development |
| Year | 2000 |
| Vol | 127 |
| Start Page | 1081 |
| Evidence for Interaction | Embryos injected with pitx2a displayed ectopic activation of pitx2a in both marginal and animal pole cells at 30% epiboly |
| Target Gene | pitx2a |
| Upstream Gene | pitx2a |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 4.66 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | H. Joseph Yost |
| Journal | Development |
| Year | 2000 |
| Vol | 127 |
| Start Page | 1081 |
| Evidence for Interaction | Embryos injected with pitx2a displayed ectopic activation of pitx2a in both marginal and animal pole cells at 30% epiboly |
| Target Gene | pitx2a |
| Upstream Gene | pitx2a |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 4.66 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | H. Joseph Yost |
| Journal | Development |
| Year | 2000 |
| Vol | 127 |
| Start Page | 1081 |
| Evidence for Interaction | Mesendoderm expression of pitx2c is dependent on spt, oep, sur and cyc. At 50% epiboly, pitx2 expression was completely absent in oep mutant embryos.At 50% epiboly, pitx2 expression was completely absent in oep mutant embryos, which display strong defects |
| Target Gene | pitx2a |
| Upstream Gene | oep |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | mesendoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | H. Joseph Yost |
| Journal | Development |
| Year | 2000 |
| Vol | 127 |
| Start Page | 1081 |
| Evidence for Interaction | At 50% epiboly, pitx2 expression was completely absent in oep mutant embryos. |
| Target Gene | pitx2a |
| Upstream Gene | oep |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | mesendoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | H. Joseph Yost |
| Journal | Development |
| Year | 2000 |
| Vol | 127 |
| Start Page | 1081 |
| Evidence for Interaction | In embryos injected with pitx2a RNA, the number of gsc expressing cells on the dorsal side was reduced |
| Target Gene | gsc |
| Upstream Gene | pitx2a |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | dorsal side |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | H. Joseph Yost |
| Journal | Development |
| Year | 2000 |
| Vol | 127 |
| Start Page | 1081 |
| Evidence for Interaction | Ectopic expression of pitx2a downregulated cyc expression in marginal cells (Table 2, Fig. 4B). However, injection of pitx2c RNA upregulated cyc expression in both marginal and animal cap cells |
| Target Gene | ndr2 |
| Upstream Gene | pitx2a |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | H. Joseph Yost |
| Journal | Development |
| Year | 2000 |
| Vol | 127 |
| Start Page | 1081 |
| Evidence for Interaction | Ectopic expression of pitx2a downregulated cyc expression in marginal cells. |
| Target Gene | ndr2 |
| Upstream Gene | pitx2a |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | H. Joseph Yost |
| Journal | Development |
| Year | 2000 |
| Vol | 127 |
| Start Page | 1081 |
| Evidence for Interaction | In spt mutant embryos at 50% epiboly, pitx2 expression was absent from superficial dorsal cells but still expressed in non-dorsal marginal cells |
| Target Gene | pitx2a |
| Upstream Gene | tbx16 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | H. Joseph Yost |
| Journal | Development |
| Year | 2000 |
| Vol | 127 |
| Start Page | 1081 |
| Evidence for Interaction | In tbx16 mutant embryos at 50% epiboly, pitx2a expression was absent from superficial dorsal cells but still expressed in non-dorsal marginal cells. |
| Target Gene | pitx2a |
| Upstream Gene | tbx16 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | H. Joseph Yost |
| Journal | Development |
| Year | 2000 |
| Vol | 127 |
| Start Page | 1081 |
| Evidence for Interaction | In pitx2a- or pitx2cinjected embryos, which displayed slowed epiboly, ntl expression was severely reduced in the notochord domain compared to uninjected embryos at 80-90% epiboly |
| Target Gene | Ntl |
| Upstream Gene | pitx2a |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | notochord domain |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | H. Joseph Yost |
| Journal | Development |
| Year | 2000 |
| Vol | 127 |
| Start Page | 1081 |
| Evidence for Interaction | In sur mutant embryos, which display posterior cyclopia similar to igu mutant embryos, pitx2 expression was absent except for a small domain in the polster |
| Target Gene | pitx2a |
| Upstream Gene | foxh1 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | H. Joseph Yost |
| Journal | Development |
| Year | 2000 |
| Vol | 127 |
| Start Page | 1081 |
| Evidence for Interaction | In embryos injected with pitx2a or pitx2c RNA, the number of gsc expressing cells on the dorsal side was reduced |
| Target Gene | gsc |
| Upstream Gene | pitx2a |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | mesoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | H. Joseph Yost |
| Journal | Development |
| Year | 2000 |
| Vol | 127 |
| Start Page | 1081 |
| Evidence for Interaction | In pitx2a-injected embryos, which displayed slowed epiboly, ntl expression was severely reduced in the notochord domain compared to uninjected embryos at 80-90% epiboly |
| Target Gene | Ntl |
| Upstream Gene | pitx2a |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 9 |
| Location of Interaction in evidence | notochord domain |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | H. Joseph Yost |
| Journal | Development |
| Year | 2000 |
| Vol | 127 |
| Start Page | 1081 |
| Evidence for Interaction | In pitx2a-injected embryos, lft2 expression was shortened along the anteroposterior axis and was not observed in cells at the margin. |
| Target Gene | lft2 |
| Upstream Gene | pitx2a |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | blastoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | H. Joseph Yost |
| Journal | Development |
| Year | 2000 |
| Vol | 127 |
| Start Page | 1081 |
| Evidence for Interaction | In pitx2a-injected embryos, lft2 expression was shortened along the anteroposterior axis and was not observed in cells at the margin. |
| Target Gene | lft2 |
| Upstream Gene | pitx2a |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | H. Joseph Yost |
| Journal | Development |
| Year | 2000 |
| Vol | 127 |
| Start Page | 1081 |
| Evidence for Interaction | In pitx2a-injected embryos, lft2 expression was shortened along the anteroposterior axis and was not observed in cells at the margin. |
| Target Gene | lft2 |
| Upstream Gene | pitx2a |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 9 |
| Location of Interaction in evidence | blastoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | H. Joseph Yost |
| Journal | Development |
| Year | 2000 |
| Vol | 127 |
| Start Page | 1081 |
| Evidence for Interaction | In sur mutant embryos, which display posterior cyclopia similar to igu mutant embryos, pitx2 expression was absent except for a small domain in the polster |
| Target Gene | pitx2a |
| Upstream Gene | foxh1 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 9 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | H. Joseph Yost |
| Journal | Development |
| Year | 2000 |
| Vol | 127 |
| Start Page | 1081 |
| Evidence for Interaction | In pitx2a-injected embryos, lft2 expression was shortened along the anteroposterior axis and was not observed in cells at the margin. |
| Target Gene | lft2 |
| Upstream Gene | pitx2a |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 9 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | H. Joseph Yost |
| Journal | Development |
| Year | 2000 |
| Vol | 127 |
| Start Page | 1081 |
| Evidence for Interaction | 27 genes that are expressed in the dorsal margin in wild type, but lack margin expression in MZoep mutants. |
| Target Gene | pitx2a |
| Upstream Gene | oep |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Schier, A. F. |
| Journal | Dev Biol |
| Year | 2007 |
| Vol | 304 |
| Start Page | 525 |