| fzd8c | Stage | Hours | ZFIN expression range | neural plate | endoderm | unspecified |
| Zygote | 1-cell | 0 | ||||
| Cleavage | 2-cell | 0.75 | ||||
| 4-cell | 1.0 | |||||
| 8-cell | 1.25 | |||||
| 16-cell | 1.5 | |||||
| 32-cell | 1.75 | |||||
| 64-cell | 2.0 | |||||
| Blastula | 124-cell | 2.25 | ||||
| 256-cell | 2.5 | |||||
| 512-cell | 2.75 | |||||
| 1k-cell | 3.0 | |||||
| High | 3.33 | |||||
| Oblong | 3.66 | |||||
| Sphere | 4.0 | |||||
| Dome | 4.33 | |||||
| 30% epiboly | 4.66 | |||||
| Gastrula | 50%-epiboly | 5.25 | ||||
| Germ-ring | 5.66 | I | I | |||
| Shield | 6.0 | |||||
| 75% epiboly stage | 8.0 | |||||
| 90% epiboly | 9.0 | |||||
| bud stage | 10.0 | I | ||||
| Segmentation | 1-4 somites | 10.33 | ||||
| 5-9 somites | 11.66 | |||||
| 10-13 somites | 14.0 | |||||
| 14-19 somites | 16.0 | |||||
| 20-25 somites | 19.0 | I | ||||
| 26+ somites | 22.0 | I | I | |||
| Pharyngula | Prim-5 | 24.0 | ||||
| Prim-15 | 30.0 | |||||
| Prim-25 | 36.0 | |||||
| High-pec | 42.0 | |||||
| Hatching | Long-pec | 48.0 | ||||
| Pec-fin | 60.0 |
| Evidence for Interaction | Concomitantly, the axial mesoderm domain, expressing gsc, is expanded at 50% epiboly. |
| Target Gene | gsc |
| Upstream Gene | fzd8c |
| Interaction Type | repress |
| Altered model | dominant negative |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | mesendoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Makoto Furutani-Seiki |
| Journal | Mechanisms of Development |
| Year | 2003 |
| Vol | 120 |
| Start Page | 477 |
| Evidence for Interaction | tbx6 expression at 80% epiboly,ntl expression at 50% epiboly. Both tbx6 and ntl expressed in non-axial mesoderm are strongly reduced in high dose fzb1 mRNA injected embryos. |
| Target Gene | ntl |
| Upstream Gene | fzd8c |
| Interaction Type | enhance |
| Altered model | dominant negative |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | mesendoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Makoto Furutani-Seiki |
| Journal | Mechanisms of Development |
| Year | 2003 |
| Vol | 120 |
| Start Page | 477 |
| Evidence for Interaction | tbx6 expression at 80% epiboly, ntl expression at 50% epiboly. Both tbx6 and ntl expressed in non-axial mesoderm are strongly reduced in high dose (250 pg) fzb1 mRNA injected embryos in K and M, respectively. |
| Target Gene | ntl |
| Upstream Gene | fzd8c |
| Interaction Type | enhance |
| Altered model | dominant negative |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | mesendoderm |
| Evidence for Direct | 3 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Makoto Furutani-Seiki |
| Journal | Mechanisms of Development |
| Year | 2003 |
| Vol | 120 |
| Start Page | 477 |
| Evidence for Interaction | tbx6 expression at 80% epiboly,ntl expression at 50% epiboly. Both tbx6 and ntl expressed in non-axial mesoderm are strongly reduced in high dose fzb1 mRNA injected embryos. |
| Target Gene | ntl |
| Upstream Gene | fzd8c |
| Interaction Type | enhance |
| Altered model | dominant negative |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Makoto Furutani-Seiki |
| Journal | Mechanisms of Development |
| Year | 2003 |
| Vol | 120 |
| Start Page | 477 |
| Evidence for Interaction | tbx6 expression at 80% epiboly, ntl expression at 50% epiboly. Both tbx6 and ntl expressed in non-axial mesoderm are strongly reduced in high dose (250 pg) fzb1 mRNA injected embryos in K and M, respectively. |
| Target Gene | ntl |
| Upstream Gene | fzd8c |
| Interaction Type | enhance |
| Altered model | dominant negative |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 3 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Makoto Furutani-Seiki |
| Journal | Mechanisms of Development |
| Year | 2003 |
| Vol | 120 |
| Start Page | 477 |
| Evidence for Interaction | tbx6 expression at 80% epiboly, ntl expression at 50% epiboly. Both tbx6 and ntl expressed in non-axial mesoderm are strongly reduced in high dose fzb1 mRNA injected embryos. |
| Target Gene | tbx6 |
| Upstream Gene | fzd8c |
| Interaction Type | enhance |
| Altered model | dominant negative |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Makoto Furutani-Seiki |
| Journal | Mechanisms of Development |
| Year | 2003 |
| Vol | 120 |
| Start Page | 477 |
| Evidence for Interaction | tbx6 expressed in non-axial mesoderm are strongly reduced in high dose (250 pg) fzb1 mRNA injected embryos. |
| Target Gene | tbx6 |
| Upstream Gene | fzd8c |
| Interaction Type | enhance |
| Altered model | dominant negative |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Makoto Furutani-Seiki |
| Journal | Mechanisms of Development |
| Year | 2003 |
| Vol | 120 |
| Start Page | 477 |
| Evidence for Interaction | tbx6 expression at 80% epiboly, ntl expression at 50% epiboly. Both tbx6 and ntl expressed in non-axial mesoderm are strongly reduced in high dose fzb1 mRNA injected embryos. |
| Target Gene | tbx6 |
| Upstream Gene | fzd8c |
| Interaction Type | enhance |
| Altered model | dominant negative |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | mesoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Makoto Furutani-Seiki |
| Journal | Mechanisms of Development |
| Year | 2003 |
| Vol | 120 |
| Start Page | 477 |
| Evidence for Interaction | tbx6 expressed in non-axial mesoderm are strongly reduced in high dose (250 pg) fzb1 mRNA injected embryos. |
| Target Gene | tbx6 |
| Upstream Gene | fzd8c |
| Interaction Type | enhance |
| Altered model | dominant negative |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | mesoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Makoto Furutani-Seiki |
| Journal | Mechanisms of Development |
| Year | 2003 |
| Vol | 120 |
| Start Page | 477 |
| Evidence for Interaction | In fzb1(fzd8c) mRNA injected embryos, the otx2 expression expanded posteriorly at the expense of the hoxa1 expressing domain, that corresponds to the hindbrain and spinal cord. At 80% epiboly, the expression domain of otx2 had expanded posteriorly in fzb1 |
| Target Gene | otx2 |
| Upstream Gene | fzd8c |
| Interaction Type | repress |
| Altered model | dominant negative |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | neuroectoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Makoto Furutani-Seiki |
| Journal | Mechanisms of Development |
| Year | 2003 |
| Vol | 120 |
| Start Page | 477 |
| Evidence for Interaction | In fzb1 mRNA injected embryos, the otx2 expression domain, that corresponds to the presumptive fore- and midbrain, expanded posteriorly at the expense of the hoxa1 expressing domain, that corresponds to the hindbrain and spinal cord . At 80% epiboly, the |
| Target Gene | otx2 |
| Upstream Gene | fzd8c |
| Interaction Type | repress |
| Altered model | dominant negative |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | neuroectoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Makoto Furutani-Seiki |
| Journal | Mechanisms of Development |
| Year | 2003 |
| Vol | 120 |
| Start Page | 477 |
| Evidence for Interaction | The presumptive spinal cord marker hnf1b is lost in the dominant negative RNA of fzd8c injected embryo. |
| Target Gene | hnf1b |
| Upstream Gene | fzd8c |
| Interaction Type | enhance |
| Altered model | dominant negative |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | presumptive brain |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Makoto Furutani-Seiki |
| Journal | Mechanisms of Development |
| Year | 2003 |
| Vol | 120 |
| Start Page | 477 |
| Evidence for Interaction | Both the presumptive hindbrain and spinal cord marker hoxa1 and the presumptive spinal cord marker vHNF1 are also strongly reduced or lost. |
| Target Gene | hnf1b |
| Upstream Gene | fzd8c |
| Interaction Type | enhance |
| Altered model | dominant negative |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | presumptive brain |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Makoto Furutani-Seiki |
| Journal | Mechanisms of Development |
| Year | 2003 |
| Vol | 120 |
| Start Page | 477 |
| Evidence for Interaction | Both the presumptive hindbrain and spinal cord marker hoxa1 and the presumptive spinal cord marker vHNF1 are also strongly reduced or lost. |
| Target Gene | hoxa1a |
| Upstream Gene | fzd8c |
| Interaction Type | enhance |
| Altered model | dominant negative |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | presumptive brain |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Makoto Furutani-Seiki |
| Journal | Mechanisms of Development |
| Year | 2003 |
| Vol | 120 |
| Start Page | 477 |
| Evidence for Interaction | The presumptive hindbrain and spinal cord marker hoxa1 is strongly reduced in fzd8c dominant negative embryos. |
| Target Gene | hoxa1a |
| Upstream Gene | fzd8c |
| Interaction Type | enhance |
| Altered model | dominant negative |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | presumptive brain |
| Evidence for Direct | 3 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Makoto Furutani-Seiki |
| Journal | Mechanisms of Development |
| Year | 2003 |
| Vol | 120 |
| Start Page | 477 |
| Evidence for Interaction | In fzb1 mRNA injected embryos, the otx2 expression domain, that corresponds to the presumptive fore- and midbrain, expanded posteriorly at the expense of the hoxa1 expressing domain, that corresponds to the hindbrain and spinal cord . At 80% epiboly, the |
| Target Gene | otx2 |
| Upstream Gene | fzd8c |
| Interaction Type | repress |
| Altered model | dominant negative |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | presumptive brain |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Makoto Furutani-Seiki |
| Journal | Mechanisms of Development |
| Year | 2003 |
| Vol | 120 |
| Start Page | 477 |
| Evidence for Interaction | In situ hybridization analysis of 10-somite stage embryos with markers revealed that the pax2.1 expression domain in the forebrain becomes enlarged. |
| Target Gene | pax2a |
| Upstream Gene | fzd8c |
| Interaction Type | repress |
| Altered model | dominant negative |
| Time of Interaction in evidence | 14 |
| Location of Interaction in evidence | ectoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Makoto Furutani-Seiki |
| Journal | Mechanisms of Development |
| Year | 2003 |
| Vol | 120 |
| Start Page | 477 |
| Evidence for Interaction | In situ hybridization analysis of 10-somite stage embryos with A-P markers revealed that the pax2.1 expression domain in the forebrain becomes enlarged. |
| Target Gene | pax2a |
| Upstream Gene | fzd8c |
| Interaction Type | repress |
| Altered model | dominant negative |
| Time of Interaction in evidence | 14 |
| Location of Interaction in evidence | brain |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Makoto Furutani-Seiki |
| Journal | Mechanisms of Development |
| Year | 2003 |
| Vol | 120 |
| Start Page | 477 |
| Evidence for Interaction | In situ hybridization analysis of 10-somite stage embryos with A-P markers revealed that the pax2.1 expression domain in the forebrain becomes enlarged. |
| Target Gene | pax2a |
| Upstream Gene | fzd8c |
| Interaction Type | repress |
| Altered model | dominant negative |
| Time of Interaction in evidence | 14 |
| Location of Interaction in evidence | brain |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Makoto Furutani-Seiki |
| Journal | Mechanisms of Development |
| Year | 2003 |
| Vol | 120 |
| Start Page | 477 |
| Evidence for Interaction | In situ hybridization analysis of 10-somite stage embryos with markers revealed that the pax2.1 expression domain in the forebrain becomes enlarged. |
| Target Gene | pax2a |
| Upstream Gene | fzd8c |
| Interaction Type | repress |
| Altered model | dominant negative |
| Time of Interaction in evidence | 14 |
| Location of Interaction in evidence | brain |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Makoto Furutani-Seiki |
| Journal | Mechanisms of Development |
| Year | 2003 |
| Vol | 120 |
| Start Page | 477 |