Expression Table

Gene Name: cyp26c1 (cytochrome P450, family 26, subfamily C, polypeptide 1 )
Description: DNA binding
Key: I = Interpolated; X = Not Detected; ? = Inconsistent data; ! = Expressed but no region
Table built from ZFIN data (http://zfin.org/zf_info/downloads.html): Tue Dec 4 14:01:03 PST 2007

cyp26c1

Stage

Hours

ZFIN expression range

deep blastomere

blastoderm

neural plate

presumptive rhombomere 2

presumptive rhombomere 3

presumptive rhombomere 4

presumptive rhombomere 5

presumptive rhombomere 6

midbrain hindbrain boundary neural keel

eye

rhombomere 2

rhombomere 4

rhombomere 5

rhombomere 6

diencephalon

hindbrain

midbrain

splanchnocranium

telencephalon

otic vesicle

retina

pectoral fin

mandibular arch

whole organism

unspecified

Zygote

1-cell

Cleavage

2-cell

0.75

4-cell

1.0

8-cell

1.25

16-cell

1.5

32-cell

1.75

64-cell

2.0

Blastula

124-cell

2.25

256-cell

2.5

512-cell

2.75

1k-cell

3.0

High

3.33

Oblong

3.66

Sphere

4.0

Dome

4.33

30% epiboly

4.66

Gastrula

50%-epiboly

5.25

Germ-ring

5.66

Shield

6.0

75% epiboly stage

8.0

90% epiboly

9.0

bud stage

10.0

Segmentation

1-4 somites

10.33

5-9 somites

11.66

10-13 somites

14.0

14-19 somites

16.0

20-25 somites

19.0

26+ somites

22.0

Pharyngula

Prim-5

24.0

Prim-15

30.0

Prim-25

36.0

High-pec

42.0

Hatching

Long-pec

48.0

Pec-fin

60.0

Literature Evidence

1. Mutant embryo and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
2. Overexpression of specific genes and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
3. Morpholino knock-down the expression of specific gene and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
4. The interaction sites of the input to downstream target genes have been identified by isolated the module and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
5. The interaction sites of the input to downstream target genes have been identified by coinject the Morpholino or over-expression the genes for reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
6. The interaction sites of the input to downstream target genes have been identified by mutation the binding sites and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)

Evidence for Interaction Inactivation of Spry2 causes an expansion of the expression domain of a marker of dorsal ectoderm (anterior neurectoderm) cyp26a and the concomitant reduction of ventral ectoderm (epidermis) revealed by foxi1 expression .

Target Gene cyp26c1

Upstream Gene spry2

Interaction Type repress

Altered model MASO

Time of Interaction in evidence 8

Location of Interaction in evidence epiblast

Evidence for Direct 3

Binding Site Sequence

Location of Site

Last Author Bernard Thisse

Journal Development

Year 2004

Vol 131

Start Page 2853

Evidence for Interaction Inactivation of Spry2 causes an expansion of the expression domain of a marker of dorsal ectoderm (anterior neurectoderm) cyp26a and the concomitant reduction of ventral ectoderm (epidermis) revealed by foxi1 expression .

Target Gene cyp26c1

Upstream Gene spry2

Interaction Type repress

Altered model MASO

Time of Interaction in evidence 8

Location of Interaction in evidence margin

Evidence for Direct 3

Binding Site Sequence

Location of Site

Last Author Bernard Thisse

Journal Development

Year 2004

Vol 131

Start Page 2853

Evidence for Interaction Inactivation of Spry2 causes an expansion of the expression domain of a marker of dorsal ectoderm (anterior neurectoderm) cyp26a and the concomitant reduction of ventral ectoderm (epidermis) revealed by foxi1 expression .

Target Gene cyp26c1

Upstream Gene spry2

Interaction Type repress

Altered model MASO

Time of Interaction in evidence 8

Location of Interaction in evidence presumptive brain

Evidence for Direct 3

Binding Site Sequence

Location of Site

Last Author Bernard Thisse

Journal Development

Year 2004

Vol 131

Start Page 2853

Evidence for Interaction Cyp26 enzymes generate the retinoic acid response pattern necessary for hindbrain development. Hnf1b is expressed in the posterior hindbrain up to the r5-r6 boundary (arrowheads) and is shifted anteriorly in Cyp26-depleted embryos.

Target Gene hnf1b

Upstream Gene cyp26c1

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 24

Location of Interaction in evidence hindbrain

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Moens, C.B.

Journal Development

Year 2007

Vol 134(1)

Start Page 177

Evidence for Interaction cyp26a1 expression domain is reduced in rbpja MO-injected embryos

Target Gene cyp26c1

Upstream Gene rbpja

Interaction Type enhance

Altered model MASO

Time of Interaction in evidence 24

Location of Interaction in evidence tailbud and posterior PSM

Evidence for Direct 3

Binding Site Sequence

Location of Site

Last Author Oates, A.C.

Journal Dev. Biol.

Year 2007

Vol 301

Start Page 388

Evidence for Interaction	Inactivation of Spry2 causes an expansion of the expression domain of a marker of dorsal ectoderm (anterior neurectoderm) cyp26a and the concomitant reduction of ventral ectoderm (epidermis) revealed by foxi1 expression .
Target Gene	cyp26c1
Upstream Gene	spry2
Interaction Type	repress
Altered model	MASO
Time of Interaction in evidence	8
Location of Interaction in evidence	epiblast
Evidence for Direct	3
Binding Site Sequence
Location of Site
Last Author	Bernard Thisse
Journal	Development
Year	2004
Vol	131
Start Page	2853

Evidence for Interaction	Cyp26 enzymes generate the retinoic acid response pattern necessary for hindbrain development. Hnf1b is expressed in the posterior hindbrain up to the r5-r6 boundary (arrowheads) and is shifted anteriorly in Cyp26-depleted embryos.
Target Gene	hnf1b
Upstream Gene	cyp26c1
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	24
Location of Interaction in evidence	hindbrain
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Moens, C.B.
Journal	Development
Year	2007
Vol	134(1)
Start Page	177

Evidence for Interaction	cyp26a1 expression domain is reduced in rbpja MO-injected embryos
Target Gene	cyp26c1
Upstream Gene	rbpja
Interaction Type	enhance
Altered model	MASO
Time of Interaction in evidence	24
Location of Interaction in evidence	tailbud and posterior PSM
Evidence for Direct	3
Binding Site Sequence
Location of Site
Last Author	Oates, A.C.
Journal	Dev. Biol.
Year	2007
Vol	301
Start Page	388